DNA Replication

1. Helicases unwind the parental double helix
   1. Single strand binding proteins stabilize the unwound parental DNA
2. DNA polymerase reads the single-strand 3′ -> 5′ and produces the leading strand 5′ -> 3′
   1. Individual nucleotides are paired together to make the new strand

3. lagging strand – Primase makes short RNA primers
   • DNA polymerase extends them to form Okazaki fragments
4. RNA primer is replaced by DNA (DNA polymerase)
   • DNA ligase joins the Okazaki fragments into a growing strand