DNA Replication

  1. Helicases unwind the parental double helix
    1. Single strand binding proteins stabilize the unwound parental DNA
  2. DNA polymerase reads the single-strand 3′ -> 5′ and produces the leading strand 5′ -> 3′
    1. Individual nucleotides are paired together to make the new strand

  1. lagging strand – Primase makes short RNA primers
    • DNA polymerase extends them to form Okazaki fragments
  2. RNA primer is replaced by DNA (DNA polymerase)
    • DNA ligase joins the Okazaki fragments into a growing strand